Skip to main content
. Author manuscript; available in PMC: 2021 Oct 19.
Published in final edited form as: Cell Chem Biol. 2021 Apr 8;28(5):648–661.e5. doi: 10.1016/j.chembiol.2021.03.011

Figure 6. Endogenous Brachyury Degradation by TRAFTACs.

Figure 6.

A) Co-immunoprecipitation of brachyury in the presence and absence of brachyury-TRAFTAC or control-TRAFTAC oligos. Cell lysates were subjected to HA immunoprecipitation and eluted samples were probed for brachyury and HT7.

B) Hela cells transiently transfected with NT-dCas9HT7 followed by a second transfection with brachyury-TRAFTAC. Then HP14 and HP17 were treated and cell lysates were prepared after 15 h and probed for brachyury, c-Myc and tubulin.

C) Hela cells stably overexpressing NT-dCas9HT7 were transfected with either with brachyury-TRAFTAC or allscrambled-TRAFTAC followed by HP14 or HP17 treatment. Cell lysates were then probed for brachyury and tubulin levels. (Not significant (N.S); * P< 0.03; ** p<0.002; *** p<0.0002; **** p<0.0001; n: Independent biological replicates; n=2)