Fig 1.

Methods and analysis. (a) Schematic showing timing of serial skin biopsies before induction of anaesthesia and 48 h after the haemodynamic trial intervention. (b)Venn diagram showing primary and secondary outcome analyses. Blue shading/text denotes patients in whom oxygen delivery (DO₂) remained low after intervention. Red shading/text denotes subjects in whom DO₂ was normal after intervention. Primary outcome was comparison between proteins differentially expressed in patients with normal vs low DO₂ after intervention. Grey shaded area highlights secondary pathway analyses which accounted for directional changes in protein expression for subjects with normal or low DO₂ value. An increase/decrease was defined by fold-change >1.5, confidence score >20 and P-value <0.05 (analysis of variance, anova), as generated by Progenesis QI analysis software (Nonlinear Dynamics, Newcastle upon Tyne, UK).