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. 2021 Oct 11;24(6):848. doi: 10.3892/mmr.2021.12488

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Copyright: © Wei et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — TRAF6, NLPR3, caspase-1 and caspase-3 mRNA and protein expression levels in pancreatic tissue. Reverse transcription-quantitative PCR analysis was performed to determine the expression levels of (A) TRAF6, (B) NLRP3, (C) caspase-1 and (D) caspase-3. (E) Western blotting was performed to determine the protein expression levels of (F) TRAF6 (58 kDa), (G) NLRP3 (118 kDa), (H) cleaved caspase-1 (12 kDa) and (I) cleaved caspase-3 (19 kDa). *P<0.05 vs. control; ^#P<0.05 vs. control and AP24H. TRAF6, tumor necrosis factor receptor-associated factor 6; NLRP3, NLR pyrin domain containing 3; AP24H, caerulein-induced acute pancreatitis for 24 h; AP48H, caerulein-induced acute pancreatitis for 48 h.