FIGURE 5.

Bacterial OMVs transfer functional Cre mRNA to multiple organs. (a–g) Representative data derived from different organ tissue of Rosa26.tdTomato mice treated with either E. coli ^Cre plus E. coli ^GFP (n = 9) or with E. coli ^GFP only (n = 5). Experiments were repeated 3 times with similar results. (a) Representative confocal images of different organ cross sections of spleen, heart and liver showing tdTomato‐positive cells (red). Nuclei counterstaining with Hoechst (blue). Scale bar: 100 μm. (b) Representative Maestro ex vivo images of the liver of Rosa26.tdTomato mice treated with E. coli ^Cre (plus E. coli ^GFP) or E. coli ^GFP as control analyzed via multi‐spectral separation. Scale bar: 50 mm. (c) Representative confocal images of kidney cross‐sections showing tdTomato‐positive cells (red). Tubular epithelium shown in green. Nuclei counterstaining with Hoechst (blue). Scale bar: 100 μm. (d) Representative pictures of tdTomato positive biliary epithelial cells in liver cross sections. Scale bar: 50 μM. Arrows point towards tdTomato‐positive cells. HPV: hepatic portal vein; HA: hepatic artery; BD: bile duct. Scale bar: 25 μm. (e‐g) Representative confocal images of immunohistochemical staining of brain cross‐section showing tdTomato‐positive cells (red) and staining against NeuN (e,g) (Green, Neuron). Nuclei counterstaining with DAPI (blue). Scale bar: 50 μm (Upper image), 10 μm (Lower image). LV: lateral ventricles; St: striatum; HC: hippocampus; Co: cortex