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. 2021 Oct 4;12:666097. doi: 10.3389/fmicb.2021.666097

Table 2.

ExoY geno-and phenotype in P. aeruginosa reference panel strains.

# Source Strain ExoY aa sequencea S. c.btoxicity Virulence from (1)c Biofilmd Motilitye Alginatef ExoYg ng/ml GCh (nmol/min/ml) GC (nmol/min/ml/OD)
L/H CFU
1 CF LES B58 100 L 7.7×103 ++ +/− − 150 40±1 24±0.1
2 CF LES 400 100 L 2.5×105 ++ − − − −
3 CF LES 431 100 L 6.6×105 + − − − −
4 CF C3719 V158L, V320L L 7.2×106 +++ − − − −
5 CF, early DK2 V158L, V320L + H 0.5 +++ + − 75 33±5 23±1.5
6 CF AES-1R I69V, D110N, E181Q, V320L, and S366G L 6.5×102 ++ +/− − − 0.6±0.05 0.7±0.04
7 CF AUS23 D110N H 2.0 +++ + − 125 43±2 22±1
8 CF AUS52 D110N, P182A H 1.0×105 +++ − − 10 4±0.01 3.1±0.1
9 CF, early AA2 P182A, S366G + H 0.4 +++ + − 285 54±4 26±1.5
10 CF, late AA43 P182A, S366G H 0.6 ++ + − 530 77±1 32±1
11 CF, late AA44 P182A, S366G H 3.8 +++ + − 560 111±3 59±2
12 CF, early AMT0023-30 D110N H 0.6 ++ + − 10 9±1 7±0.7
13 CF, late AMT0023-34 D110N, frameshift P242 − L 2.6×105 +++ +/− −
14 CF, late AMT0060-1 R193W + H 2.0 +++ + − 60 27±5 18±2
15 CF, late AMT0060-2 R193W L 3.1×103 + +/− + − 1.1±0.2 0.7±0.2
16 CF, early AMT0060-3 R193W H 0.8 +++ + − 90 21±0.1 12±0.2
17 Non CF PAO1 100 + H 0.3 +++ + − 500 94±5 57±3
18 Non CF UCBPP-PA14 D110N, R141Q, S227N, V320L, and frameshift F373 + H 2.1 +++ + − 10 0.4±0.2 0.3±0.2
19 Non CF PAK D110N, R141Q, E181Q, and V320L + H 1.1 +++ + − 450 75±2 31±1
20 CF CHA D110N, E181Q, and V320L H 2.6 +++ + + − −
22 CF IST27 D110N, E181Q, and V320L H 0.4 +++ + + 25 15±3 11±3
23 CF IST27N D110N, E181Q, and V320L H 0.4 +++ + − 25 20±2 11±1.5
24 Non CF 968333S 100 L 2.5×105 +++ − + 9±0.1 9±0.1
25 Non CF 679 100 H 4.9 +++ + − 39±3 24±1.5
26 Non CF 39016 L21Q, D110N, R141Q, and frameshift 242 H 0.5 +++ + −
27 CF 2192 379 codons V320L L 1.3×104 +++ + + − −
28 CF NH57388A 100 L 5.1×103 +++ + −
29 Non CF 1709-1 R139W, frameshift P242 H 0.4 +++ + −
30 Non CF Mi 162 R193W, frameshift P242 H 0.8 ++ +/− −
31 Water Jpn 1563 V320L H 1.9 ++ + − 25 46±1 28±3
32 Water LMG 14084 L21Q, D110N, R141Q, and frameshift 242 H 0.3 + + −
33 Hospital environment Pr335 V320L, Q368L H 0.8 ++ + − 29±1 18±2
34 CF U018a D110N, E181Q, and V320L H 0.4 ++ + − 14±0.1 11±0.4
35 Tobacco plant CPHL 9433 19 replacements + H 0.5 ++ + −
36 CF RP1 D110N, E181Q H 0.3 ++ + − 150 38±17 19±9
37 Non CF 15108/-1 Pseudogenei, D110N, R141Q, G159W, R239K, and V320L H 0.5 ++ + − − −
38 Non CF 57P31PA P203L H 2.1 +++ + − 25 28±0.2 15±1
39 Non CF 13121/-1 100 H 0.2 +++ + − 450 73±1 45±6
40 Non CF 39177 V320L, Q168R H 0.6 +++ + − 110 45±1 14±0.3
41 CF KK1 P182A, S366G + H 0.5 +++ + − 250 60±9 29±4
42 Non CF A5803 Δaa 17-20, D110N, S227N, and frameshift P242 H 1.5 ++ + −
43 CF TBCF10839 100 H 0.2 ++ + − 90 52±2 31±1.5

Empty cells correspond to values that were not determined. Cells are color coded according to Figure 2.

a

100 indicates 100% identity with the aa sequence of PAO1.

b

Toxicity in S. cerevisiae: results according to Supplementary Figure 2; +, toxic, −, no toxicity.

c

Virulence in G. mellonella according to Cullen et al. (2015) summarized as H, high virulence for LD50<5CFU; L, low virulence for LD50>650 CFU; CFU indicates average values of LD50.

d

Values for the ability to form biofilms according to Ref (1). The authors point out that all strains formed biofilms to various degrees depending on medium and time. We reported values for the medium (MH, LB or M63) and the time point (24h, 48h, 72h) that produced maximal biofilms summarized as +, ++, or +++ for absorbance at 570 nm of <0.35, 0.35–0.95, or >0.95, respectively according to values in Supplementary Table 3 of Cullen et al. (2015).

e

Motility according to Table 1 of Cullen et al. (2015); −, loss of motility; +/−, only swimming motility; +, at least 2 modes of motility detected out of 3 (swimming, swarming, twitching).

f

Alginate production according to Table 1 of Cullen et al. (2015); +, production of high levels of alginate; −, low level or no alginate production.

g

− Indicate protein amounts below the detection limit in western blots.

h

− Indicate GC activities <0.2 nmol/min/ml.

i

The exoY allele from strain #37 is annotated as pseudogene in Pseudomonas.org (2), the aa sequence aligns to that of PAO1 starting from aa 50.