FIG. 6.

CAF-1 interacts with specific sites on the outer front side of PCNA. (A) Front and side views of the homotrimeric PCNA toroid showing positions of the mutations used in the GST pull-down assay. (B) GST pull-down of wild-type and mutant recombinant human PCNAs by GST-p150(1-649). Equivalent amounts of C-terminally histidine-tagged wild-type (WT-His) and mutant (LAPK251, QLGI125, SHV43, and VDK188; created via alanine substitutions) PCNA proteins were used in each experiment. Input (I), unbound (U), bound (B), 0.6 M KCl elution (E), and 0.6 M KCl-resistant (B*) fractions of PCNA proteins were revealed by Western blotting with a monoclonal antibody against PCNA.