. 2021 Sep 23;10(10):2579–2591. doi: 10.1021/acssynbio.1c00224

Table 2. Strains and Plasmids Use in This Work.

strain	characteristic	references
Escherichia coli TOP-10	F-, mcrA, Δ(mrr-hsdRMS-mcrBC),φ80lacZΔM15, ΔlacX74, nupG, recA1, araD139, Δ(ara-leu)7697, galE15, galK16, rpsL(StrR), endA1, λ-	Thermo Fisher Scientific
pUC57-SyncM	Amp^R, syncM synthetic gene cloned under P_nis promoter	this work
pUC57-SyncA1–2	Amp^R, synthetic genes for syncA1 and syncA2	this work
pUC57-SyncA3–6	Amp^R, synthetic gene for prochlorosins syncA3, syncA4, syncA5, and syncA6	this work
pUC57-SyncA7–10	Amp^R, synthetic gene for prochlorosins syncA7, syncA8, syncA9, and syncA10	this work
pUC57-SyncA11–14	Amp^R, synthetic gene for prochlorosins syncA11, syncA12, syncA13, and syncA14	this work
pUC57-SyncA15–18	Amp^R, synthetic gene for prochlorosins syncA15, syncA16, syncA17, and syncA18	this work
Lactococus lactis NZ9000	pepN:nisRK	(53)
pTLR	Ery^R, E. coli-L. lactis shuttle vector	Solmeglas, Madrid, Spain⁵⁴
pNZ8048	Cm^R	(40)
pNZ8048-SyncA (1–18)	Each syncA genes cloned in pNZ8048, under P_nis promoter	this work
pNZe-NisP8H	Ery^R, Cm^R. NisP producer strain	(41)
pTLR-SyncM	Ery^R, syncM cloned in pTLR under P_nis promoter	this work
pTLR-SyncM pNZ8048-SyncA(1–18)	Ery^R, Cm^R, 18 different strains with the different syncA genes (1–18) cloned individually under P_nis promoter in pNZ8048 in L. lactis NZ9000 pTLR-SyncM	this work