Table 3. USER Primers for Cloning of SyncM and SyncM to pTLR and pNZ8048, Respectivelya.
| name | sequence |
|---|---|
| pTLR-user-rv | AGATCACGAGCUCGCGAAAGCTTGTTGTAACTGAAAAAG |
| pTLR-user-fw | AAGCATGCGGUGTCGACCGATGCCCTTGAGAGCCTTCAAC |
| Pnis-user-fw | AGCTCGTGATCUAGTCTTATAACTATACTGACAATAGAAAC |
| LanM-USER-rv | ACCGCATGCTUCTGCTTTTTGGCTATCATTCAAGCTTAACACGTG |
| pNZ-user-rv | ATGGTGAGTGCCUCCTTATAATTTATTTTG |
| pNZery-user-fw | AAGCATGCGGUCTTTGAACCAAAATTAGAAAACCAAGGCTTG |
| Proc33-USER-fw | AGGCACTCACCAUGAGCCATCATCATCATCATCATCACGAA |
| SyncA2-USER-fw | AGGCACTCACCAUGAGCCATCACCACCATCATCATCATTTAGAACAAC |
| ProcA-USER-rv | ACCGCATGCTUAACACGTGAATTTGTTTAATTGCCATTTCAATTG |
a
Italic letters are the homology between neighboring fragments, and at the 3′ end of the homology region is an uracil residue.