Figure 1.

Cell-free expression of BtubA/B. (A) Schematic of a bacterial microtubule consisting of the BtubA/B subunits arranged in four to five protofilaments. The protein BtubC binds to the outside of the filament, predominantly interacting with BtubB. (B) Schematic of CFPS reaction. PUREfrex2.0 was supplemented with the GreenLys reagent to fluorescently label the synthesized proteins through boron dipyrromethene (BODIPY)-conjugated lysine residues. (C) SDS-PAGE analysis of the gene expression products. The gene names of the expressed DNA templates are indicated on the lanes. The uncropped gel is shown in Figure S2.