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. 2021 Oct 1;22(6):1400. doi: 10.3892/etm.2021.10836

Figure 2.

Figure 2

Effect of CoCl2 and SFR on the vitality of H9c2 cardiac myoblasts was detected using a CCK-8 assay. (A) H9c2 cardiac myoblasts were cultured with different concentrations of CoCl2 for 24 h. **P<0.01 vs. CONT. (B) H9c2 cardiac myoblasts were cultured with different concentrations of SFR for 12 h. (C) Effect of reoxygenation time on the vitality of H9c2 cardiac myoblasts. *P<0.05 vs. Reoxygenation 0 h group. (D) The vitality of H9c2 cardiac myoblasts in CONT, H/R, SFRL, SFRH, SFRA and LY294002 + SFRH + H/R groups was detected using a CCK-8 assay. Data are presented as the mean ± SEM. n=6. **P<0.01 vs. CONT; ##P<0.01 vs. H/R; and ΔΔP<0.01 vs. SFRL, low dose SFR; SFRH, high dose SFR; SFRA, SFR alone. SFRH + H/R. SFR, safranal; H/R, hypoxia/reoxygenation; CONT, control; CCK-8, Cell Counting Kit-8.