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. 2021 Oct 1;22(6):1400. doi: 10.3892/etm.2021.10836

Figure 8.

Figure 8

Effect of SFR on the protein expression levels of Bax, Bcl-2, caspase-3 and cleaved caspase-3. (A) Western blot analysis of the Bcl-2, Bax, capase-3 and cleaved caspase-3 expression levels in CONT, H/R, SFRL + H/R and SFRH + H/R groups. (B) The ratio of Bcl-2 to Bax expression levels normalized to β-actin. (C) Quantification of the Bcl-2 and Bax protein expression levels normalized to β-actin. Quantification of the (D) capase-3 and (E) cleaved caspase-3 protein expression levels normalized to β-actin. (F) Western blot analysis of Bax, Bcl-2, capase-3 and cleaved caspase-3 in CONT, H/R, SFRL + H/R, SFRH + H/R, SFRA and LY294002 + SFRH + H/R groups. (G) Quantification of the Bcl-2, Bax, capase-3 and cleaved caspase-3 protein expression levels normalized to β-actin. Data are presented as the mean ± SEM. n=3 **P<0.01 vs. CONT; #P<0.05, ##P<0.01 vs. H/R; and ΔP<0.05, ΔΔP<0.01 vs. SFRH + H/R. SFRL, low dose SFR; SFRH, high dose SFR; SFRA, SFR alone. SFR, safranal; H/R, hypoxia/reoxygenation; CONT, control.