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Primer sequences used for reverse transcription-quantitative PCR.
| Gene | Primer | Sequence | Product size (bp) |
|---|---|---|---|
| NgR | Forward | 5'-ACCCTCCCATGTAGACGTCTCC-3' | 637 |
| Reverse | 5'-CTGAAGAGCAATGTTGTTTAGC-3' | ||
| CSPG | Forward | 5'-GATGGAATCTTTCACCCAAGAA-3 | 960 |
| Reverse | 5'-ACCGAGTTGAGCTTTGTCCAAC-3 | ||
| β-actin | Forward | 5'-GGCACCACACCTTCTACAAT-3' | 961 |
| Reverse | 5'-TAGCCTTGGCGAGCAACGGT-3' |
NgR, Nogo receptor; CSPG, chondroitin sulfate proteoglycan.
