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. 2021 Oct 18;12:6071. doi: 10.1038/s41467-021-26282-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A, B Monocle3-based pseudotemporal analysis of CD8⁺ subsets. C–F Expression of GZMA, GZMB, GZMK and GNLY in cells projected onto the trajectory of CD8⁺ continuum. G Pearson correlation between GZMK and other cytotoxic genes in CD8⁺ cells. H Heatmap of differentially expressed genes among CD8⁺ T lymphocyte subsets. I UMAP of MAIT cells with exhaustion and cytotoxicity scores projection. J Distribution of T-cell subsets in PT1. K Heatmap of differentially expressed genes between MAIT subsets.