Fig. 2.
Enzymatic activity of CD73 and CD38 on peripheral blood B cells.
a-e B cells (a-b, e) and T cells (c-d) from the peripheral blood of healthy controls (HC) and SLE patients (SLE) were isolated. Purine concentrations in cell supernatants (1×105 cells / sample) were assessed by HPLC after addition of 20 µM AMP (a, c) or 20 µM NAD (b, d) at the indicated time points or without addition of exogenous purines after incubation for 10 min (e). f Purine concentrations in blood serum of HC and SLE patients analysed by HPLC. Means ± SD (a: HC n=9, SLE n=15; b: HC n=6, SLE n=13; c: HC n=5, SLE n=6; d: HC n=5, SLE n=6; e: HC n=3, SLE n=4; f: HC n=5, SLE n=5 donors). *P<0·05, **P<0·01, ***P<0·001 [Multiple t tests]. n.d., not detected.