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. 2021 Oct 18;21:1073. doi: 10.1186/s12879-021-06751-y

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Effects of IL-27 on IL-18 and IL-18bp gene expression and release from hemozoin-exposed endothelial cells. Human aortic endothelial cells were primed with recombinant (rh)IL-27 (100 ng/mL, 1 h) and incubated with 10, 50 and 100 μg/mL hemozoin (Hz) for 22 h. IL-18 (A) and IL-18bp (B) were measured in supernatants from the cells with EIA. Gene expression analyses for IL-18 (C) and IL-18bp (D) were analyzed by qPCR, related to reference gene β-actin/TaqMan reference probes and normalized to unstimulated cells (US). Results are representatives of minimum three experiments and data are presented as mean and SEM. ***p < 0.001 versus US (white bar), ^††p < 0.01 and ^†††p < 0.001 versus Hz (blue bars) and ^###p < 0.001 versus IL-27 (hatched bars)