Fig. 1.

The effect of PKC on OAT1 transport function. OAT1-expressing COS-7 cells were treated for 1 h, with 10 μM PMA in the absence or presence of 2 μM staurosporine (St), or 2 μM St alone. 4-min uptake of [³H]-para-aminohippuric acid (PAH, 20 μM) was then measured at room temperature. Each data point represents OAT1-mediated transport only, after subtracting uptake values from cells transfected with empty vector. Uptake value in each group is expressed as a percentage of the uptake value measured in control cells. Values are mean ± S.D. (n = 3). *p < 0.05