Fig. 5.

Per-site performance of 5mC prediction. A Correlation plot showing Pearson correlation coefficients of each methylation-calling tool with BS-Seq on NA19240. The white squares in the upper triangle show Pearson correlation coefficients. Cross-platform (i.e., between BS-seq and nanopore sequencing) correlation coefficients are in red while cross-tool (i.e., pair-wise comparison between each methylation-calling tool for nanopore sequencing) correlation coefficients are in black. The density plots in the diagonal exhibit the distributions of 5mC percentage. The blue squares in the lower triangle represent the 2D kernel density plots for each pair of comparisons. B Relationship between CpG methylation percentage and distance to annotated TSS in the NA19240 (left panel) and NA12878 (right panel) datasets. Distances are binned into 50-bp windows. C Relationship between CpG methylation percentage and distance to annotated CTCF binding peaks in the NA19240 (left panel) and NA12878 (right panel) datasets. Distances are binned into 100-bp windows for NA19240 and 125-bp windows for NA12878. Negative distances are upstream and positive distances are downstream of the B TSS or C CTCF binding peaks