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. 2021 Oct 18;11:183. doi: 10.1186/s13578-021-00695-1

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — NMB expression during chondrogenesis. A Detection of NMB (13 KDa) and β-actin (45 KDa) proteins in BM-MSCs at d0 and micropellets at d3 by Western blotting (left panel) and their respective quantification (right panel). B Relative expression of NMB and NMBR in BM-MSCs from healthy and OA donors quantified by RT-qPCR. Results are expressed as mean ± sem (n = 3 biological replicates). C Chondrogenic differentiation of BM-MSCs characterized by increased gene expression of SOX9, collagen type IIB (COL2A1varB), aggrecan (ACAN) and collagen type X (COL10A1) at d21 compared to d0 (left panel) (n = 6 biological replicates). Kinetics of NMB expression during chondrogenesis of BM-MSCs isolated from healthy (middle panel) or OA (right panel) donors and cultured with or without TGFβ3 (n = 3 biological replicates by group of donors). D Correlation between NMB expression in BM-MSCs at d0 and chondrogenic markers (SOX9, COL2A1varB, ACAN, COL10A1) at d21 of chondrogenic differentiation (n = 17). *: p ≤ 0.05, **: p ≤ 0.01