TABLE 1.
Base sequences of the staphylococcal toxin-specific oligonucleotide primers and predicted sizes of amplified products
| Genea | GenBank accession no. | Primer | Oligonucleotide sequence (5′→3′) | Size of amplified productb (bp) | Reference |
|---|---|---|---|---|---|
| sea | M18970 | SEA-1 | TTGGAAACGGTTAAAACGAA | 120 | 12 |
| SEA-2 | GAACCTTCCCATCAAAAACA | ||||
| seb | M11118 | SEB-1 | TCGCATCAAACTGACAAACG | 478 | 12 |
| SEB-2 | GCAGGTACTCTATAAGTGCC | ||||
| sec | X05815 | SEC-1 | GCATAAAAGCTAGGAATTT | 257 | 12 |
| SEC-2 | AAATCGGATTAACATTATCC | ||||
| sed | M28521 | SED-1 | CTAGTTTGGTAATATCTCCT | 317 | 12 |
| SED-2 | TAATGCTATATCTTATAGGG | ||||
| see | M21319 | SEE-1 | CAAAGAAATGCTTTAAGCAATCTTAGGCCAC | 482 | 12 |
| SEE-2 | CTTACCGCCAAAGCTG | ||||
| seg | AF064773 | SEG-1 | AATTATGTGAATGCTCAACCCGATC | 642 | This study |
| SEG-2 | AAACTTATATGGAACAAAAGGTACTAGTTC | ||||
| seh | U11702 | SEH-1 | CAATCACATCATATGCGAAAGCAG | 375 | This study |
| SEH-2 | CATCTACCCAAACATTAGCACC | ||||
| sei | AF064774 | SEI-1 | CTCAAGGTGATATTGGTGTAGG | 576 | This study |
| SEI-2 | AAAAAACTTACAGGCAGTCCATCTC | ||||
| tst | J02615 | TSST-1 | ATGGCAGCATCAGCTTGATA | 350 | 12 |
| TSST-2 | TTTCCAATAACCACCCGTTT | ||||
| eta | M17347 | ETA-1 | CTAGTGCATTTGTTATTCAA | 119 | 12 |
| ETA-2 | TGCATTGACACCATAGTACT | ||||
| etb | M17348 | ETB-1 | ACGGCTATATACATTCAATT | 200 | 12 |
| ETB-2 | TCCATCGATAATATACCTAA |
a
Genes encoding SEA to SEE, SEG to SEI, TSST-1, ETA, and ETB.
b
Size of PCR product derived from published GenBank sequences.