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CRISPR Toolset for Genetic Screens
| DNA Binding Protein | DNA Cleavage? | Effector | Mechanistic Result | Assess the Role of |
|---|---|---|---|---|
| Cas9 | Yes | None | Loss-of-function (knockout) | Protein-coding genes, miRNAs, enhancers, … |
| dCas9 | No | None | Transcriptional repression | All genes |
| KRAB (CRISPR interference) | Transcriptional repression | All genes | ||
| VP16, VP64 (CRISPR activation) | Transcriptional activation | All genes |
Abbreviation: dCas9, dead Cas9.
