Figure 2.
Calcium homeostasis modulates the TLR5 response. (A) Histamine-dependent cytosolic Ca2 + responses in differentiated airway epithelial cells pretreated (red) or not (blue) with 100-ng/ml apical FliCΔ174–400 for 24 hours and stimulated with histamine (100μM) for 1 minute. Differences were evaluated by using the Kolmogorov-Smirnov (K-S) test. (B) Boxplot showing the fold change increase in the histamine-induced peak Ca2 + response in cells pretreated or not with FliCΔ174–400 for 24 hours. “Basal” indicates the mean value before histamine stimulation. (C) Quantitative PCR analysis of differentiated airway epithelial cells from wild-type pigs pretreated or not with 1μM thapsigargin for 24 hours and later stimulated or not with flagellin (100 ng/ml) for 5 hours. “Mock” indicates PBS-treated and nonstimulated airway epithelial cells. Gene expression is shown relative to the mock group. Intergroup differences were analyzed by using the Kruskal-Wallis test followed by a pairwise Mann-Whitney test with Benjamini-Hochberg false discovery rate correction. Data are representative of three experiments. a.u.= arbitrary unit; ns = not significant.