Table 1:
Primary antibodies.
| Antibody | Supplier (Catalog No.) | Antigen | Host (clone) | Dilution (diluent) |
|---|---|---|---|---|
| β-Actin | Proteintech (#66009-1-Ig) | human ACTB | mouse (2D4H5) | 1:1,000 (5% milk powder) |
| DELE1 | Novus Bio. (#NBP2-84765) | human DELE1 (aa 164-213) | rabbit (polyclonal) | 1:500 (5% milk powder) |
| OMA1 | Santa Cruz Biotech. (#sc-515788) | human OMA1 (aa 230-399) | mouse (H-11) | 1:250 (5% milk powder) |
| OPA1 | BD Biosciences (#612606) | human OPA1 (aa 708-830) | mouse (18/OPA-1) | 1:1,000 (5% milk powder) |
| P-Tau S199/S202 | ThermoFisher (#44-768G) | human TAU phosphopeptide around aa 199-202 | rabbit (polyclonal) | 1:1,000 (3% BSA) |
| P-Tau S396 | Novus Bio. (#NB100-82243) | human TAU phosphopeptide around aa 396 | rabbit (polyclonal) | 1:1,000 (3% BSA) |
| P-Tau S396 (PHF13) | Santa Cruz Biotech. (#sc-32275) | human PHF-Tau | mouse (PHF13) | 1:250 (3% BSA) |
| P-Tau S404 | Cell Signaling Tech. (#20194) | human tau phosphorylated at S404; S400 & S404; T403 & S404; S400, T403 & S404 | rabbit (D2Z4G) | 1:500 (3% BSA) |
| P-Tau T231 | Novus Bio. (#NB100-82249) | human tau phosphopeptide around aa 231 | rabbit (polyclonal) | 1:1,000 (3% BSA) |
| PGAM5 | Proteintech (#28445-1-AP) | human PGAM5 isoform 2 | rabbit (polyclonal) | 1:1,000 (5% milk powder) |
| Phosphoserine | Novus Bio. (#NB100-1953) | phosphorylated serine residues | rabbit (polyclonal) | 1:100 (3% BSA) |
| Phosphothreonine | Novus Bio. (#NB110-96896) | phosphorylated threonine residues | rabbit (polyclonal) | 1:125 (3% BSA) |
| PINK1 | Novus Bio. (#BC100-494) | human PINK1 (aa 175-250) | rabbit (polyclonal) | 1:1,000 (5% milk powder) |
| Tau | EMD Millipore (#MABN2472 ) | phosphorylation-independent N-terminal region | mouse (2A1-2E1) | 1:1,000 (5% milk powder) |