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Gene mutagenesis at GAD PAM sites induced by xCas9 in rice T0 plants. (A) Schematic illustration of the pxCas9 construct for the CRISPR-xCas9 system. (B) Mutation types at GAA-1 and GAT-1 target sites of some representative stable mutant lines. Ref sequence is the wild-type sequence. Target sequences are underlined and PAM sequences are indicated in red. (C) Frequencies of different mutation types at GAD PAM sites mutated by xCas9.
