Table 1.
Summary of the mutation frequencies and mutation types at GA PAM sites targeted by xCas9.
| PAM sequence | Target site | Target gene | Target sequence | Tested T0 plants | Edited T0 plants | Mutation frequency (%) | Mutation types |
|---|---|---|---|---|---|---|---|
| GAA | GAA-1 | OsMPK2 | CCCTTCATGAGATATATGATGAA | 17 | 5 | 29.4 | 4He+1Ho |
| GAA-2 | OsMPK5 | CACCTTCAACCCGCTGCAGAGAA | 19 | 0 | 0 | ||
| GAA-3 | OsNRT1.1B | ACCAGGAGGTACAGGTTCAAGAA | 18 | 0 | 0 | ||
| GAT | GAT-1 | OsMPK5 | GCCAAGCGCACGCTCCGGGAGAT | 20 | 15 | 75 | 7He+4Bi+1Ho+3Chi |
| GAT-2 | OsWaxy | CTCATGCAGGAGGACGTCCAGAT | 20 | 2 | 10 | 1He+1Bi | |
| GAT-3 | OsWaxy | CTGCTCCTTGAAGAGCCTGAGAT | 20 | 1 | 5 | 1He | |
| GAG | GAG-1 | OsWaxy | CGTCATTCCTGGAGAAGGTGGAG | 20 | 13 | 65 | 11He+1Bi+1Chi |
| GAG-2 | OsALS | CCAACCACCTCTTCCGCCACGAG | 20 | 1 | 5 | 1He | |
| GAG-3 | OsALS | GCTTCCTCATGAACATTCAGGAG | 20 | 9 | 45 | 9He | |
| GAC | GAC-1 | OsWaxy | TACCGAGGAGAGATCACCATGAC | 20 | 0 | 0 | |
| GAC-2 | OsGRF4 | CACTTTCGTTCTTTGGGAACGAC | 20 | 0 | 0 | ||
| GAC-3 | OsNRT1.1B | GCGACCACCATCATGTTCTGGAC | 20 | 0 | 0 | ||
| GAC-4 | OsALS | ACCTTGTCCTTGATGTGGAGGAC | 20 | 0 | 0 | ||
| GAC-5 | OsWaxy | CCACCGGCTTCGGCATCGCCGAC | 20 | 0 | 0 | ||
| GAC-6 | OsALS | ACCTCGTGTCCGCGCTCGCCGAC | 20 | 0 | 0 | ||
| GAC-7 | OsNRT1.1B | GCTTCGGCTCCGACCAGTTCGAC | 20 | 0 | 0 | ||
| GAC-8 | OsPDS | TCCTGCAGAGGAATGGGTTGGAC | 20 | 0 | 0 |
The PAM sequence is underlined. He, heterozygous mutation; Bi, biallelic mutation; Ho, homozygous mutation; Chi, chimeric mutation.