Table 2.
Summary of the mutation frequencies and mutation types at NG PAM sites targeted by xCas9.
| PAM sequence | Target site | Target gene | Target sequence | Tested T0 plants | Edited T0 plants | Mutation frequency (%) | Mutation types |
|---|---|---|---|---|---|---|---|
| NGG | NGG-1 | OsMPK2 | ACACTGCAGCTATTGATATCTGG | 20 | 19 | 95 | 1He+10Bi+4Ho+4Chi |
| NGG-2 | OsMPK5 | CGACATGATGACGGAGTACGTGG | 20 | 9 | 45 | 3He+1Bi+5Chi | |
| NGA | NGA-1 | OsGRF4 | GCATTCTCATCAGCGAGGTCTGA | 20 | 14 | 70 | 4He+1Bi+9Chi |
| NGA-2 | OsNRT1.1B | GCTCTACCTGGGGCTCTACCTGA | 19 | 5 | 26.3 | 5He | |
| NGT | NGT-1 | OsMPK2 | CAACGCCCGCAGATATGTGAGGT | 19 | 18 | 94.7 | 3He+10Bi+5Ho |
| NGT-2 | OsMPK5 | CATCCGCTCCAACCAAGAACTGT | 19 | 13 | 68.4 | 7He+2Bi+1Ho +3Chi | |
| NGC | NGC-1 | OsMPK5 | TCAGGCCGACGATGACGCACGGC | 20 | 0 | 0 | |
| NGC-2 | OsMPK2 | AGACCTCAGGCCAAGTAATTTGC | 20 | 0 | 0 | ||
| NGC-3 | OsWaxy | GGCACACTGGCCCACTGGCGAGC | 20 | 0 | 0 | ||
| NGC-4 | OsMPK5 | AGCCGCCCATCATGCCCATTGGC | 20 | 6 | 30 | 5He+1Chi | |
| NGC-5 | OsMPK2 | CCACCTTCTTCGATCAAACCAGC | 20 | 0 | 0 | ||
| NGC-6 | OsWaxy | TCGGCCACCGGCTTCGGCATCGC | 20 | 0 | 0 |
The PAM sequence is underlined. He, heterozygous mutation; Bi, biallelic mutation; Ho, homozygous mutation; Chi, chimeric mutation.