Table 3.
C-to-T mutations induced by xCas9n-CBE in rice T0 plants.
| PAM sequence | Target site | Tested T0 plants | Edited T0 plants | C-to-T frequency (%) | Genotypes | Homozygous |
|---|---|---|---|---|---|---|
| GAA | GAA-1 | 12 | 4 | 33.3 | C2>T2(1); C2C3>T2T3(1); C1C2C3>T1T2T3(2) |
0 |
| GAA-2 | 12 | 0 | 0 | |||
| GAA-3 | 12 | 0 | 0 | |||
| GAT | GAT-1 | 19 | 9 | 47.4 | C3>T3(3); C2C3>T2T3(6) |
2 |
| GAT-2 | 20 | 0 | 0 | |||
| GAT-3 | 20 | 0 | 0 | |||
| GAG | GAG-1 | 20 | 14 | 70 | C4>T4(10); C1C4>T1T4(3); C4C9>T4T9(1) |
2 |
| GAG-2 | 20 | 2 | 10 | C15>T15(2) | 0 | |
| GAG-3 | 20 | 4 | 20 | C2>T2(2); C2C5>T2T5(2) |
0 | |
| NGG | NGG-1 | 19 | 13 | 68.4 | C2>T2(1); C2C4>T2T4(10); C2C4C7>T2T4T7(2) |
3 |
| NGG-2 | 20 | 5 | 25 | C4>T4(5) | 1 | |
| NGA | NGA-1 | 20 | 13 | 65 | C2>T2(12); C2C6C8>T2T6T8(1) |
4 |
| NGA-2 | 19 | 1 | 5.3 | C4>T4(1) | 0 | |
| NGT | NGT-1 | 18 | 12 | 66.7 | C1>T1(1); C4>T4(5); C7>T7(2); C1C4>T1T4(1); C4C7>T4T7(1); C6C7>T6T7(1); C1C7C10>T1T7T10(1) |
0 |
| NGT-2 | 20 | 10 | 50 | C5>T5(1); C4C5>T4T5(8); C4C5C10>T4T5T10(1) |
0 | |
| NGC | NGC-4 | 27 | 14 | 51.9 | C3C4>T3T4(13); C3C4C8>T3T4T8(1) |
0 |
| NGC-5 | 29 | 0 | 0 | |||
| NGC-6 | 30 | 0 | 0 | |||
| GAC | GAC-1 | 19 | 2 | 10.5 | C3>T3(1); C3C4>T3T4(1) |
0 |
| GAC-2 | 20 | 0 | 0 | |||
| GAC-3 | 20 | 1 | 5 | C2>T2(1) | 0 |