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. 2021 Aug 11;3:717017. doi: 10.3389/fgeed.2021.717017

FIGURE 1.

FIGURE 1

Protoplast regeneration and CRISPR genome editing. (A) Protoplast regeneration-related articles according to taxonomy. These 779 protoplast regeneration articles were identified from Google scholar and NCBI (Supplementary Table S1). The taxonomy of the plant species follows the Angiosperm Phylogeny Group IVsystem and NCBI taxonomy. Major familes are color coded as follows: purple; Asteraceae, light orange; Solanaceae, green; Brassicaceae, orange; Rutaceae, blue; Fabaceae, yellow-green; Poaceae. The names of the species reported in more than five articles are shown. The rest of the species are shown in grey. The information used to create this diagram is shown in Supplementary Table S1. (B) Schematic of tobacco protoplast regeneration. Delivery methods stated in grey are the methods used for protoplast transformation that can theoretically be applied in genome editing. The multiple peaks (black arrows in “Efficiency assessment” and “Progeny genotyping”) in the upstream of PAM (green boxes) are indicated. tThe target site (red boxes) is editied by CRISPR reagent.