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. 2020 May 28;2:3. doi: 10.3389/fgeed.2020.00003

Figure 2.

Figure 2

Weight gain and immunophenotype of NHE1 W38−/− birds. (A) Representative photo of sibling chicks at 2 weeks of age: NHE1 W38+/+ (left) and modified NHE1 W38−/− (right). (B) Growth curve of NHE1 W38+/+ and NHE1 W38−/− birds. Weight gain was measured weekly from the first day of hatch until 14 weeks of age (n ≥ 5). (C) Immunophenotype of NHE1 W38+/+ and NHE1 W38−/− chickens at 2 weeks of age. Data display relative amounts of T-cells (γδ T-cells: NHE1 W38+/+ 1,5%; NHE1 W38−/− 1,8% and αβ T-cells: NHE1 W38+/+ 8,0%; NHE1 W38−/− 9,3%), B-cells (NHE1 W38+/+ 0,9%; NHE1 W38−/− 2,0%) and monocytes (NHE1 W38+/+ 1,7%; NHE1 W38−/− 1,3%), of peripheral blood mononuclear cells (PBMCs) measured by flow cytometry (n ≥ 5). Error bars indicate standard deviation (SD). Statistical tests were performed using two-sided Student's t-test compared to NHE1 W38+/+ birds (p > 0.05).