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. 2020 May 28;2:3. doi: 10.3389/fgeed.2020.00003

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Copyright © 2020 Hellmich, Sid, Lengyel, Flisikowski, Schlickenrieder, Bartsch, Thoma, Bertzbach, Kaufer, Nair, Preisinger and Schusser.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Resistance of NHE1 W38^−/− mutants to ALV-J compared to NHE1 W38^+/+. Infection experiment of NHE1 W38^+/+ and NHE1 W38^−/− chicken embryonic fibroblasts with RCAS(J)eGFP. Infection of the eGFP expressing virus was assessed by microscopy on 14 dpi (A) and by flow cytometry on 2 and 7 dpi (B,C). (A) Microscopy shows a representative replicate of infected NHE1 W38^+/+ and NHE1 W38^−/− CEFs (Scale bar = 100 μm). (B) Flow cytometrical data on 7 dpi. CEFs were gated depending on side- and forward scatter (SSC/FSC) and examined for eGFP fluorescence intensity. Upper panels show infected NHE1 W38^+/+ and lower panels display NHE1 W38^−/− CEFs. (C) Comprehensive data of eGFP quantification by flow cytometry on 2 and 7 dpi. Black dots represent NHE1 W38^+/+ (2 dpi 5.5%; 7 dpi 8.1%) and blue dots NHE1 W38^−/− (2 dpi and 7 dpi 0%). Infections were carried out in triplicates in three independent experiments for the indicated cell lines. Standard deviation (SD) is indicated by error bars. Statistical analysis was performed using Mann–Whitney-U-Test (*p < 0.05).