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. 2020 Jun 4;2:5. doi: 10.3389/fgeed.2020.00005

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Copyright © 2020 Zafar, Sedeek, Rao, Khan, Amin, Kamel, Mukhtar, Zafar, Mansoor and Mahfouz.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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General strategy for improving rice through genome editing. The first step is gRNA design and construct development. After confirmation of the construct it is transferred either in Agrobacterium or coated on gold particles for biolistic bombardment. The construct is transformed to rice callus. The plants are regenerated from this callus. The regenerated plants are confirmed through PCR and restriction digestion, followed by sequencing. The plants are then screened for desired characteristics and moved to the greenhouse and then to field trials.