Figure 5.

Development of transgene-free genetically edited rice plants for commercialization (A). The gRNA will be designed for targeted modification and during construct development appropriate transgene-free method will be chosen (e.g., transient expression of Cas9, RNP or suicide transgene method etc.) (B). For genome editing, the appropriate genome editing tool will be chosen depending upon the feasibility and type of modification that needs to be introduced. (C). The developed construct can be delivered to plant by Agrobacterium, biolistic or transfection. (D). Once the construct is delivered into the plant. It will perform its function by modifying the target site in the rice genome. After modification the transgene will be removed from the plants. (E). The regenerated plants will be screened for targeted modification without transgene. The plants developed in this way will be with desired traits without any transgene integration in the genome, so can be labeled as NON-GMO genetically edited plants by regulatory authorities.