Figure 1.

Schematic presentation of nanoblades. (A) Representation of the different components of the nanoblades: gag-pol, the gag-Cas9 fusion protein, the different gRNA and the envelope glycoproteins (gps), (B) Schematic representation of the assembly of a nanoblade particle, (C) Scheme of the MLV GAG:Cas9 fusion (left) and HIV GAG:Cas9 fusion (right) indicating the inserted MLV or HIV protease site followed by a Flag tag. (D) Schematic representation of the mutant BaEV envelope gp (BaEVRless) and the VSV-G envelope gp. The R-peptide of the cytoplasmic tail of BaEVwt was deleted resulting in the BaEVRLess mutant gp.