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. 2021 Feb 9;3:604371. doi: 10.3389/fgeed.2021.604371

Figure 2.

Figure 2

BaEV/VSV-G gp pseudotyped nanoblade-mediated gene editing in cell lines. (A) Concentration of Cas9 in nM present in the nanoblades measured by ELISA in MLV and HIV-derived nanoblades co-pseudotyped with BaEV- and VSVG-gps (B + V) or pseudotyped only with BAEV-gp or VSVG-gp (V) (means ± SD, n = 3; student t-test, *p < 0.05 and **p < 0.01) (B) Schematic representation of expected band sizes of WAS PCR product with WASFw/WASRv primers before the cleavage (811 bp), which represent the intact WAS gene and the edited WAS gene after the deletion upon DSB at both gRNA targets (647 bp); the gRNA-301 and−305 target sites are indicated as a black and red gRNA, respectively; (C) Graph summarizing percentage of cleavage (right) (means ± SD; MLV B + V n = 17, MVL B n = 4, MLV V n = 5, HIV B + V n = 16, HIV B n = 7, HIV V n = 6; student t-test, *p < 0.05, **p < 0.01, and ***p < 0.001). (D) Representative electrophoresis gel of the PCR products on genomic DNA from 293T cells transduced with MLV or HIV nanoblades co-pseudotyped with B + V, B, or V gps; quantification of gene editing is indicated for each lane (left).