Skip to main content
. 2021 Feb 9;3:604371. doi: 10.3389/fgeed.2021.604371

Figure 4.

Figure 4

BaEV/VSV-G gp displaying nanoblades allow efficient gene-editing in hB cells. (A) Raji cells transduced with MLV- or HIV-based nanoblades co-pseudotyped with BaEV- and VSVG-gps (B + V). Electrophoresis gel of the PCR product using WASFw/WASRv primers with the % gene-editing indicated for each lane (left) and summarizing graph showing percentage of gene editing (right) (means ± SD; MLV B + V n = 4, HIV B + V n = 5), (B) Raji cells were treated with polybrene (PB) or with polybrene and Retronectin (PB+R) and then incubated with MLV nanoblades co-pseudotyped with B + V. Electrophoresis gel of the PCR product using WASFw/WASRv primers with the % gene-editing indicated for each lane (left) Graph showing percentage of gene editing (right) (means ± SD; MLV B + V n = 3, HIV B + V n = 3; student t-test, **p < 0.01, n.s., not significant). (C) Primary human B cells preactivated with Pansorbin A and IL-2 were incubated with MLV or HIV nanoblades co-pseudotyped with BaEV- and VSVG-gps (B + V). Representative gel of the PCR product using WASFw/WASRv primers with the percentage of gene-editing indicated for each lane (left) and graph showing percentage of gene editing (right) (means ± SD MLV B + V n = 3, HIV B + V n = 3; student t-test, n.s., not significant).