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. 2021 Mar 3;3:644319. doi: 10.3389/fgeed.2021.644319

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Copyright © 2021 Meneghini, Peviani, Luciani, Zambonini and Gritti.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CRISPR/Cas9 delivery platforms and routes of administration. A list of advantages (green) and drawbacks (red) of viral (lentiviral and adeno-associated vectors) and non-viral (nanoparticles) delivery methods. Troubleshooting methods (blue) to overcome potential drawbacks are reported. The delivery routes of viral vectors and nanoparticles are indicated, highlighting their advantages (green) and limitations (red). IP, intraparenchymal; ICV, intracerebroventricular; IT, intrathecal; IV, intravenous; CRISPR, clustered regularly interspaced short palindromic repeats.