(A and B) The expression level of PRR11 in ccRCC cell lines (ACHN, 796-P, 786-O, and Caki-1 cells) and a human renal tubular epithelial cell line (HK2; n = 3). (C) PRR11 staining score of ccRCC tissues and adjacent tissues based on a tissue microarray (n = 89 ccRCC tissues and n = 89 adjacent tissues). (D) Correlation between the PRR11 staining score and the ccRCC pathological grade based on a tissue microarray (n = 32 for grade I, n = 31 for grade II, n = 25 for grade III, and n = 1 for grade IV). (E) Representative pattern of PRR11 protein expression in ccRCC tissues and adjacent tissues using tissue microarray sections. (F) Survival analysis of PRR11 expression based on a ccRCC tissue microarray. Scale bar: 400 μm (left) and 100 μm (right). The data are shown as mean ± SD. One-way ANOVA with Dunnett’s multiple comparisons test (A), 2-tailed t test (C), and 1-way ANOVA with Tukey’s multiple comparisons test (D) analyses were performed. *P < 0.05, **P < 0.01, ***P < 0.001. PRR11, proline rich 11; ccRCC, clear cell renal cell carcinoma.