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. 2021 Oct 8;6(19):e145172. doi: 10.1172/jci.insight.145172

Figure 8. E2F1 played an important role in the PRR11-mediated regulation of the proliferation and migration of ccRCC cells.

Figure 8

(A–D) Transwell and clonogenic assays were used to analyze the effect of E2F1 knockdown on the migration and colony formation of ACHN cells transfected with shPRR11 (n = 3). Scale bar: 150 μm. (E and F) E2F1 knockdown significantly reversed the effect on the cell cycle progression of shPRR11-transfected ACHN cells (n = 3). (G) MTT assay confirmed that E2F1 knockdown significantly enhanced the proliferation of ACHN cells transfected with shPRR11 (n = 3). (H–J) The expression of PRR11, E2F1, cell cycle–related genes, and migration-related genes in ccRCC cells was determined by Western blotting. The data are shown as mean ± SD. Two-way ANOVA with Tukey’s multiple comparisons test analyses were performed (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001. ccRCC, clear cell renal cell carcinoma; PRR11, proline rich 11.