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. 2021 Oct 8;6(19):e152109. doi: 10.1172/jci.insight.152109

Figure 5. RNA deep sequencing and qPCR.

Figure 5

Relative expression of the lipid metabolism gene LPCAT3 in the central (left; CR) and peripheral (right; PR) retina, analyzed by RNA deep sequencing (top) or qPCR (bottom). Similar changes were observed by both methods. In the top panels, n is 6, 6, and 4 for participants without diabetes, diabetes without DR, and diabetes with DR, respectively. In the bottom panel n is, 22, 22, and 20 for participants without diabetes, diabetes without DR, and diabetes with DR, respectively. qPCR P values are adjusted by sex, hypertension, hyperlipidemia, coronary artery disease, nephropathy, and cancer. The statistical test was ANOVA. Data are shown as mean ± SD (*P < 0.05 in DR versus ND).