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. 2021 Oct 8;6(19):e146089. doi: 10.1172/jci.insight.146089

Figure 7. Adoptive transfer of M2-polarized macrophages to dams reduces inflammatory cytokines in maternal peripheral blood and fetal amniotic fluid.

Figure 7

(A) M2-polarized macrophages (M2 Mɸ) or vehicle were i.v. administered on 15.5 dpc and 16.5 dpc to C57BL/6 dams followed by intra-amniotic injection with LPS on 16.5 dpc (M2+LPS). Control C57BL/6 dams were injected intra-amniotically with LPS only (LPS). Dams were euthanized 16 hours after LPS injection to collect maternal plasma and fetal amniotic fluid. Multiplex bead assays were used to determine the concentrations of CCL2 (B), CCL4 (C), CXCL2 (D), CXCL10 (E), IL-5 (F), IFN-γ (G), G-CSF (H), and IL-10 (I) in plasma (n = 10 dams per group) and concentrations of CXCL10 (J) and IL-10 (K) in amniotic fluid (n = 5–8 each). Data are presented as box plots where midlines indicate medians, boxes indicate IQR, and whiskers indicate minimum/maximum range. Symbols are values from individual dams and fetuses. Data were analyzed by Mann-Whitney U tests.