Figure 1.

Effect of NaOx exposure on proliferation activity of MDCK cells. (A) Cells were treated with various doses (0, 0.5, 1, 2, and 4 mmol/L) of NaOx for 24 h, followed by a determination of cell proliferation activity assessed by trypan blue staining. (B) The activity of lactate dehydrogenase (LDH) released into medium from cell treated with NaOx for 24 h was detected. (C and D) Following treatment with NaOx for 24 h, the confluent monolayers were wounded with pipette tips. The cell migration rate was recorded at 0, 12, and 24 h, respectively. Images are representative of three observations. Scale bar indicates 50 μm. Values are means ± SEM. n = 3. Means without common superscripts differ (P < 0.05). Abbreviations: MDCK, Mardin–Darby canine kidney; NaOx, sodium oxalate.