Figure 3.

Sodium oxalate-induced stagnation in cell proliferation evaluated by EdU assay and cell cycle-related gene expression. (A) MDCK cells were allowed to proliferate for 24 h in the presence of different doses of NaOx, followed by EdU and Hoechst 33342 staining. Scale bar: 20 μm. (B) Cells were treated with NaOx for 24 h before RNA extraction, after which RT-qPCR was performed to detect the relative mRNA level of CCND1 and PCNA. GAPDH was used as a reference gene (n = 3). Means without a common letter differ (P < 0.05). Abbreviations: Edu, 5-ethynyl-2′-deoxyuridine; MDCK, Mardin–Darby canine kidney; NaOx, sodium oxalate; RT-qPCR, real-time quantitative PCR.