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The addition of NaOx inhibits the expression of tight junction proteins at both transcriptional and protein levels. Cells treated with 0, 0.5, 1, 2, and 4 mmol/L of NaOx for 24 h were harvested for RT-qPCR (A) or Western blot analysis (B and C). GAPDH was regarded as a reference gene or loading control (n = 3). Means without a common letter differ (P < 0.05). Abbreviations: NaOx, sodium oxalate; RT-qPCR, real-time quantitative PCR.
