Figure 3.

Dose response curves for mitochondrial median circularity and length using 4-hydroxychalcone and 2,4-dihydroxychalcone. Each compound was assayed over a concentration range from 1 to 10 μM on two duplicate plates. Robust z-scores were calculated for mitochondrial median circularity and length values of the neuritic mitochondria for each well and were plotted against the log₁₀ of the molar concentrations of the compounds. Each point on the plot represents the robust z-score average±SEM of 8 independent wells between the two independent plates. High concentrations of each compound caused toxicity in the wells as illustrated by increased and/or decreased z-scores (light grey points) where appropriate. These values were excluded for calculating the EC₅₀ values.