Table 1.
i3Neuron CAG>mtTagGFP2 lentivirus MOI titration day 7 results for 15,000 cells per well cell density
| parameter | MOI | day | average* | SEM | ANOVA** |
|---|---|---|---|---|---|
| cell body count | 1 | 7 | 1.2 | 0.3 | p value = 0.0002 |
| cell body count | 3 | 7 | 5.3 | 0.6 | NS |
| cell body count | 5 | 7 | 7.5 | 0.4 | |
| cell body count | 7 | 7 | 11.0 | 0.3 | p value = 0.0012 |
| cell body count | 10 | 7 | 12.0 | 0.9 | p value < 0.0001 |
|
| |||||
| CA | 1 | 7 | 96 | 26 | p value < 0.0001 |
| CA | 3 | 7 | 465 | 24 | p value < 0.0001 |
| CA | 5 | 7 | 735 | 52 | |
| CA | 7 | 7 | 940 | 39 | p value = 0.0030 |
| CA | 10 | 7 | 1230 | 31 | p value < 0.0001 |
|
| |||||
| mito count | 1 | 7 | 90 | 25 | p value < 0.0001 |
| mito count | 3 | 7 | 425 | 22 | p value < 0.0001 |
| mito count | 5 | 7 | 676 | 46 | |
| mito count | 7 | 7 | 853 | 36 | p value = 0.0041 |
| mito count | 10 | 7 | 1122 | 25 | p value < 0.0001 |
|
| |||||
| median circularity | 1 | 7 | 0.59 | 0.15 | NS |
| median circularity | 3 | 7 | 0.79 | 0.00 | NS |
| median circularity | 5 | 7 | 0.78 | 0.00 | |
| median circularity | 7 | 7 | 0.77 | 0.00 | NS |
| median circularity | 10 | 7 | 0.77 | 0.00 | NS |
|
| |||||
| median length | 1 | 7 | 1.03 | 0.27 | p value = 0.0349 |
| median length | 3 | 7 | 1.55 | 0.01 | NS |
| median length | 5 | 7 | 1.55 | 0.01 | |
| median length | 7 | 7 | 1.58 | 0.02 | NS |
| median length | 10 | 7 | 1.59 | 0.01 | NS |
CA = sum of the total mitochondrial area; NS = not significant; MOI = multiplicity of infection.
*
4 fields were imaged per well and 7 independent wells were tested per MOI; field data was collapsed into an average well value for each variable tested; the 7 well values were averaged together to provide the data in the table above.
**
ANOVA results illustrate whether the parameter value at a particular MOI was significantly different from that same parameter value at a MOI=5.