Table 2.
i3Neuron CAG>mtTagGFP2 lentivirus MOI titration day 8 results for 15,000 cells per well cell density
| parameter | MOI | day | average* | SEM | ANOVA** |
|---|---|---|---|---|---|
| cell body count | 1 | 8 | 1.5 | 0.4 | p value < 0.0001 |
| cell body count | 3 | 8 | 6.1 | 0.4 | p value = 0.0440 |
| cell body count | 5 | 8 | 8.9 | 0.8 | |
| cell body count | 7 | 8 | 11.8 | 0.6 | p value = 0.0283 |
| cell body count | 10 | 8 | 13.1 | 1.0 | p value = 0.0009 |
|
| |||||
| CA | 1 | 8 | 114 | 31 | p value < 0.0001 |
| CA | 3 | 8 | 591 | 30 | p value = 0.0002 |
| CA | 5 | 8 | 953 | 73 | |
| CA | 7 | 8 | 1247 | 56 | p value = 0.0029 |
| CA | 10 | 8 | 1686 | 54 | p value < 0.0001 |
|
| |||||
| mito count | 1 | 8 | 111 | 31 | p value < 0.0001 |
| mito count | 3 | 8 | 561 | 29 | p value = 0.0001 |
| mito count | 5 | 8 | 916 | 69 | |
| mito count | 7 | 8 | 1166 | 54 | p value = 0.0078 |
| mito count | 10 | 8 | 1605 | 47 | p value < 0.0001 |
|
| |||||
| median circularity | 1 | 8 | 0.58 | 0.15 | NS |
| median circularity | 3 | 8 | 0.79 | 0.00 | NS |
| median circularity | 5 | 8 | 0.79 | 0.00 | |
| median circularity | 7 | 8 | 0.78 | 0.00 | NS |
| median circularity | 10 | 8 | 0.78 | 0.00 | NS |
|
| |||||
| median length | 1 | 8 | 1.06 | 0.27 | NS |
| median length | 3 | 8 | 1.54 | 0.01 | NS |
| median length | 5 | 8 | 1.53 | 0.01 | |
| median length | 7 | 8 | 1.56 | 0.01 | NS |
| median length | 10 | 8 | 1.55 | 0.01 | NS |
CA = sum of the total mitochondrial area; NS = not significant; MOI = multiplicity of infection.
*
4 fields were imaged per well and 7 independent wells were tested per MOI; field data was collapsed into an average well value for each variable tested; the 7 well values were averaged together to provide the data in the table above.
**
ANOVA results illustrate whether the parameter value at a particular MOI was significantly different from that same parameter value at a MOI=5