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. 2021 Oct 8;6(19):e150551. doi: 10.1172/jci.insight.150551

Figure 3. Mutagenesis analyses of cysteine-311 and cysteine-471 regarding formation of intermolecular disulfide bond(s).

Figure 3

(A–C) SEC analyses of rIgA in the presence of TCEP, DTT, or glutathione, respectively. (D–F) SEC tracing of C471S, C311/471S, and C311 mutations of rIgA as compared with wild-type, respectively. The bar graphs show quantitation of poly- versus mono-rIgA contents based on AUC. (G) Comparing HEK293-produced human rIgA (hu-rIgA) wild-type and C471S mutant in terms of their poly-hu-rIgA contents as revealed by SEC.