Skip to main content
. 2021 Oct 8;6(19):e147038. doi: 10.1172/jci.insight.147038

Figure 6. High INSIG1 level is responsible for the ER retention of SREBPs in ASGR1 deficiency.

Figure 6

In mice, (A) INSIG1 and INSIG2 protein contents were analyzed by Western blots in liver tissues, n = 3. (B) mRNA expression level of Insig1 and Insig2 in liver tissues (WT, n = 6; Asgr1+/–, n = 3; Asgr1–/–, n = 6). In HepG2 cells, (C) representative images of immunofluorescence staining for INSIG1 and ER marker PDI (scale bar: 10 μm). (D) SREBP and INSIG1 protein contents of ER fraction isolated from HepG2 cells were analyzed by Western blots. Representative images of the immunoblotting shown, n = 3. All data are shown as the means ± SD. *P < 0.05, as compared with the indicated WT by 1-way ANOVA among 3 groups. Statistical significance between 2 groups was assessed with an unpaired, 2-tailed t test.