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. 2021 Oct 8;6(19):e148887. doi: 10.1172/jci.insight.148887

Figure 7. The patient BM expressed high levels of IgG transcripts and contained abundant Ab-secreting cells.

Figure 7

Real-time PCR was performed to analyze transcript levels of individual VH genes of the BM IgM (A), peripheral IgM (B), peripheral IgG (C), and BM IgG (D) from an HC (A) or 3 HCs (B–D) and the patient. For comparison, transcript level of each VH gene in HCs was set to 1. ACTIN was used as an internal control. (E) FACS analysis of the CD38+ plasma cells in the BM of the patient and 2 HCs. (F) Real-time PCR analysis of PRDM1 (BLIMP1) and SDC1 expression in BM cells from 3 HCs and the patient. Mean ± SD is shown. (G) ELISPOT assay of IgM- and IgG-secreting plasma cells in the BM of an HC and the patient. (H and I) Mean ± SD of the numbers and sizes of the spots of triplicate wells. Statistical analyses were performed using Student’s t test. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.