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. 2021 Oct 8;6(19):e147716. doi: 10.1172/jci.insight.147716

Figure 4. Blocking mTORC1 with rapamycin attenuates alternative complement pathway activation and podocyte injury in STZ-induced diabetic mice.

Figure 4

(A) The strategy for STZ injection and rapamycin administration. (B) UACR among different groups. n = 5. (C) Representative immunohistochemical staining images for p-S6, CFB, C3d, C5b-9, and C5aR in glomerulus among different groups. Representative PAS staining and immunofluorescence staining for diabetic kidney injury, WT1, and nephrin among different groups. Scale bar: 20 μm. (D–J) Quantitative analyses of p-S6, CFB, C3d, C5b-9, C5aR, and WT1-positive podocytes per glomerulus and glomerular area among different groups. *P < 0.05, n = 4–7. Data are expressed as the mean ± SEM. Comparison between the groups was performed using the 2-tailed Student’s t test (unpaired t test).