TABLE 1.

The detailed characteristics of bZIPs identified in Glycyrrhiza uralensis.

Gene name	Protein length (aa)	MW (kDa)	pI	Instability index (II)	Aliphatic index	GRAVY	Stable yes/no a	Subcellular location
GubZIP1	457	50.66	6.47	43.47	79.02	−0.482	No	nucl
GubZIP2	424	46.23	5.91	58.88	57.85	−0.794	No	E.R.
GubZIP3	533	59.57	8.61	63.40	62.80	−0.809	No	plas
GubZIP4	463	51.14	8.53	56.99	71.97	−0.616	No	nucl
GubZIP5	788	85.39	5.74	49.41	62.98	−0.627	No	plas
GubZIP6	320	36.13	6.14	66.27	54.94	−0.977	No	plas
GubZIP7	472	52.39	8.30	57.34	78.60	−0.511	No	nucl
GubZIP8	157	17.65	6.92	52.81	83.82	−0.499	No	cyto
GubZIP9	556	60.84	6.48	58.75	58.83	−0.837	No	plas
GubZIP10	167	18.85	9.37	64.14	60.24	−0.985	No	plas
GubZIP11	144	16.51	6.83	73.60	81.94	−0.678	No	nucl
GubZIP12	360	41.15	6.48	52.51	80.81	−0.421	No	nucl
GubZIP13	145	16.41	7.87	54.46	86.90	−0.632	No	E.R.
GubZIP14	514	54.98	5.91	54.52	53.75	−0.818	No	plas
GubZIP15	156	17.38	5.90	50.70	71.41	−0.612	No	nucl
GubZIP16	342	36.26	7.68	52.18	52.31	−0.830	No	vacu
GubZIP17	215	24.40	6.89	62.61	68.98	−0.989	No	plas
GubZIP18	299	32.85	5.11	46.45	68.16	−0.656	No	plas
GubZIP19	451	49.38	8.78	52.14	77.69	−0.495	No	mito
GubZIP20	557	61.74	6.69	68.37	52.39	−1.009	No	plas
GubZIP21	435	46.64	6.82	52.39	58.39	−0.830	No	plas
GubZIP22	434	46.71	9.42	43.15	60.94	−0.680	No	nucl
GubZIP23	345	37.71	7.15	59.05	62.20	−0.810	No	E.R.
GubZIP24	331	35.30	9.07	50.86	48.94	−0.735	No	E.R.
GubZIP25	303	31.92	4.97	59.28	46.20	−0.768	No	E.R.
GubZIP26	345	38.40	5.87	69.00	69.65	−0.741	No	plas
GubZIP27	196	22.49	6.55	56.12	76.07	−0.687	No	vacu
GubZIP28	509	57.09	8.88	59.87	73.26	−0.649	No	plas
GubZIP29	362	41.20	6.27	57.73	80.11	−0.488	No	nucl
GubZIP30	483	53.54	5.93	55.30	76.81	−0.578	No	nucl
GubZIP31	666	76.37	8.77	46.34	82.66	−0.567	No	nucl
GubZIP32	245	28.15	10.16	44.91	61.67	−1.335	No	E.R.
GubZIP33	368	42.38	6.14	67.61	60.16	−1.183	No	plas
GubZIP34	132	15.68	10.16	61.21	84.92	−0.559	No	cyto
GubZIP35	202	22.82	9.69	54.95	51.14	−1.259	No	vacu
GubZIP36	338	37.90	5.03	60.28	83.96	−0.372	No	nucl
GubZIP37	328	35.90	6.34	39.51	53.54	−1.107	No	nucl
GubZIP38	527	58.76	6.29	66.54	72.47	−0.623	No	plas
GubZIP39	221	25.65	5.97	70.18	67.47	−0.985	No	nucl
GubZIP40	143	16.27	9.00	52.09	77.83	−0.683	No	nucl
GubZIP41	144	16.89	9.13	66.40	83.26	−0.756	No	nucl
GubZIP42	170	19.57	9.12	69.61	66.59	−0.712	No	E.R.
GubZIP43	347	38.51	7.90	54.06	77.90	−0.329	No	plas
GubZIP44	197	22.94	10.18	55.48	83.25	−0.652	No	nucl/cyto
GubZIP45	415	45.34	9.79	50.55	61.83	−0.832	No	nucl
GubZIP46	324	35.94	8.50	59.78	67.44	−0.810	No	nucl
GubZIP47	534	59.10	7.03	72.28	67.17	−0.759	No	plas
GubZIP48	504	55.97	6.33	49.14	75.89	−0.497	No	E.R.
GubZIP49	386	42.79	9.43	58.25	72.05	−0.721	No	E.R.
GubZIP50	404	43.86	6.43	61.02	66.86	−0.683	No	nucl/mito
GubZIP51	201	23.15	5.97	77.57	68.36	−0.885	No	plas
GubZIP52	241	27.07	7.80	67.50	75.27	−0.868	No	plas
GubZIP53	320	33.79	6.70	47.16	63.09	−0.560	No	E.R.
GubZIP54	326	36.25	6.10	59.36	69.23	−0.715	No	plas
GubZIP55	177	20.99	9.63	67.25	58.42	−1.136	No	E.R.
GubZIP56	411	45.14	9.28	59.35	62.82	−0.857	No	plas
GubZIP57	382	41.10	5.96	46.34	57.30	−0.769	No	plas
GubZIP58	176	19.64	5.37	76.73	58.75	−0.706	No	mito
GubZIP59	376	40.44	6.19	54.69	62.87	−0.717	No	E.R.
GubZIP60	276	31.00	5.40	60.86	92.93	−0.393	No	nucl
GubZIP61	313	34.83	6.62	49.53	69.17	−0.794	No	cyto
GubZIP62	165	18.44	9.09	66.27	71.52	−0.940	No	nucl
GubZIP63	196	22.51	5.65	61.84	73.11	−0.734	No	E.R.
GubZIP64	394	44.90	6.85	57.73	63.17	−0.911	No	nucl/mito
GubZIP65	334	36.54	7.08	48.12	65.69	−0.776	No	E.R.
GubZIP66	412	44.11	5.78	57.17	53.30	−0.902	No	plas

Note. aa, amino acid; MW, molecular weight; pI, isoelectric point; GRAVY, grand average of hydropathicity; nucl, nucleus; E.R., endoplasmic reticulum; plas, plasma; cyto, cytoplasm; vacu, vacuole; mito, mitochondria.

^aA protein whose instability index is smaller than 40 is predicted as stable, while above 40 predicts that the protein may be unstable (Gasteiger et al., 2005).